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Ethics Statement A study protocol was approved by the Ethics Review Committee of Aga Khan University hospital

Ferroptosis regulates lipid peroxidation via the GPX4/acyl-CoA synthetase long chain family member 4 axis, and targeting of GSH metabolism selectively eliminates resistant subpopulations that are dependent on antioxidant defenses

The main limitations of our study is the relatively small number of samples

NRF2 expression was markedly decreased in NSC-34 motor neuron cells transfected with the human SOD1 G93A gene, leading to significantly increased levels of MDA, GSH, and ROS, along with reduced expression of SLC7A11 and GPX4