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FIGURE 4 The slr0236 , sll1545 , and slr0236-sll1545 Mutants Exposed to Methylene Blue Undergo a Transient Sequential Accumulation of ROS, and Then of Reduced and Oxidized Glutathione The effects of methylene blue on the content of ROS and glutathione were similar but lower in amplitude than those triggered by the high-light stress (Figure 5), in agreement with the fact that singlet oxygens generated by MB ( per se by the ROS-detecting DCHF-DA probe (Figure 5B)