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After washed with PBS twice, cells were harvested by trypsin digestion and resuspended in PBS plus 5% FBS and analyzed by flow cytometry

Lactobacillus amylovorus inhibits the TLR4 inflammatory signaling triggered by enterotoxigenic Escherichia coli via modulation of the negative regulators and involvement of TLR2 in intestinal Caco-2 cells and pig explants