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(2) although iron chelators and antioxidants have been used to modulate ferroptosis, their therapeutic efficacy remains inadequate, limiting their clinical utility

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The primary antibodies used were: anti-MOG (1:500, AB32760, Abcam), anti-MBP (1:300, MCA409S, BioRad), anti-APCCC1 (1:50, OP80, Sigma-Aldrich), anti-PDGFR (1:50, AF1062, R&D Systems), anti-OLIG2 (1:200, MABN50, Sigma-Aldrich), anti-Ki67 (1:500, 14569882, Thermo Fisher), anti-IBA1 (1:500, 234004, SYSY), anti-GFAP (1:500, 130300, Thermo Fisher), anti-GPX4 (1:200, ab25066, Abcam), anti-HMGB1 (1:200, ab18256, Abcam), anti-MAP2 (1:500, 822501, BioLegend), anti-SYN1 (1:500, D12G5, Cell Signaling Technology), anti-SHANK3 (1:500, 162304, Synaptic Systems), anti-EAAT1 (1:50, ab181036, Abcam), anti-ALDH1A1 (1:200, AF5869, R&D Systems), anti-C1Q (1:500, ab182451, Abcam), anti-C3 (1:500, 11862, Abcam), anti-RUNX1 (1:100, sc-365644, Santa Cruz Biotechnology), anti-MOBP (1:50, Thermo Fisher), and anti-PVALB (1:300, ab11427, Abcam), anti-ANKG (1:500, 386005, SySy)

Hence we used an alternative approach where we analyzed the oxidized protein derivatized with Au 15 SG 12 -Ao after subsequent degradation of the grafted NC and trypsin digestion, whereby the putative oxime bond between the aminooxy on the NC and carbonylated amino acids on the protein remained intact

Frequently Asked Questions What is 5-Amino-1MQ studied for in research

eIF4E, eukaryotic translation initiation factor 4E