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PubMed: 10906314 GH Receptor-Independent Lipolytic Activity of AOD 9604 in Growth Hormone Receptor-Deficient Mice animal Hefti FF, Frazier-Higgins D, Duong TT, et al

Multiple validated approaches exist, with selection depending on specific goals, injury characteristics, and individual response patterns

The honest answer is that we don't know for certain

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The potential for skewed results, failed experiments, and completely non-reproducible data is enormous

First, all possible deamidations of N/Q to D/E are simulated up to the number detected by search software and ignoring N-terminal Q if the pyroGlu modification was detected, next reverse deamidation permutations of D/E to N/Q are simulated, ignoring previously altered positions and N-terminal E if the pyroGlu modification was detected, and then if N-term pyroGlu formation was detected, Q to E or E to Q substitutions are simulated depending on starting amino acid