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Withdrawal behaviors, adapted from Barr et al., 28,32,33 included paw movement, rolling, stretching, body curls, and mild and severe tremors (Table 1), were observed for 30-s intervals every 5 min, and recorded on a checklist

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The cells were then blocked with 1% bovine serum albumin (BSA) for 1 h and reacted with rabbit monoclonal antibody against GPX1 (1:200, Proteintech, USA) overnight at 4 C

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Methyl 2-(1H-indol-3-yl)acetate is an indole ester

The earliest documented use of Rhizoma coptidis , a source of berberine, for medicinal purposes dates back to the year 200 A.D (Zhang et al., 2020), kidney fibrosis ( 2 Berberine 2.1 Chemical and physical properties of BBR BBR is readily extracted from various traditional medicinal plants, where it is broadly dispersed across the roots, stems, and bark of species with notable pharmacological value