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These biomarker clusters, in turn, formed 7 groups based on fold changes of free carnitine, selected acylcarnitines, the branched chain amino acids, lactate, and pyruvate and reveal patterns of metabolic dysregulation in autism

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To get an estimate of the CAT activity, GR activity, and GPx activity, absorbance was measured at 570 nm, 340 nm, and 420 nm, respectively.[ , , ] Steroid hormones assay The serum levels of testosterone hormone (TH), luteinizing hormone (LH), and follicle-stimulating hormone (FSH) were analyzed utilizing rat-specific ELISA kits that were purchased from Qayee-Bio Life Science, China, and the assays were performed based on the competitive binding principle