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10.1038/nature02661 165 LuJ.XuY.HuW.GaoY.NiX.ShengH.et al

Key Takeaway: Glutathione serves as the bodys primary defense against oxidative stress

Begin with small doses, get feedback, and course correct as you go

They may be reconstituted in a small amount of a basic solvent such as 0.1% aqueous ammonia and then diluted with water to the desired concentration

doi: 10.1016/j.bbagen.2012.11.020 16

Recombinant protein purification and in vitro kinase assay Full-length human G6PD (wild-type, 2YA or 2YF mutant) sequences were cloned into the pGEX-4T1 vector and expressed as the GST-tagged fusion proteins in E