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TREATMENT Early initiation of treatment is critical immediately following recognition of DILI

Materials and Methods Protein expression and purification GST-tagged GATE16 was expressed from the plasmid pGex-4T-2_GATE-16 (73518

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The mean fluorescence intensity of CellROX was significantly higher in immune cells from TLS than those from the spleen or peripheral blood (Supplemental Figure 4)

Khler ES, Sankaranarayanan S, van Ginneken CJ, van Dijk P, Vermeulen JL, Ruijter JM, Lamers WH, Bruder E (2008) The human neonatal small intestine has the potential for arginine synthesis

Cells were harvested and centrifuged at 1200 rpm for 3 min, followed by incubation with 1 L annexin V-Alexa Fluor 488 (640906, BioLegend) in 100 L annexin V binding buffer 1 (10 mM HEPES (pH 7.4), 0.14 M sodium chloride (NaCl), 2.5 mM calcium chloride (CaCl 2 ) and incubated at room temperature in the dark for 15 min