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Similarly, the expression of a constitutively activated form of GSK3 (p.S9A mutant) in HepG2 cells induced a significant decrease of H 2 S production (Figure 2G) without affecting cell proliferation and/or viability (Supplementary Figure 1E)

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Determination of vesicle size, polydispersity index, and zeta-potential The average particle size, polydispersity index (PDI), and zeta potential of the fabricated ivermectin nanoformulations were determined using a Malvern Zetasizer Nano series ZS instrument (Malvern Instruments, Malvern, UK) after appropriate dilution at room temperature 37