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Jun Guo participated in the manuscript writing

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Popcorn pellets were collected by centrifugation for 30 min at 4000 g, resuspension in 100 L of ice-cooled lysis buffer [50 mM Tris pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM MgCl 2 , 0.5% NP40 (Research Products International), 125 U/mL Benzonase (Millipore), 1x protease inhibitor cocktail (Roche, cOmplete ULTRA tablets, mini, EDTA-free), 0.5 mg/ml lysozyme (Fisher Scientific)], followed by dropwise addition into liquid nitrogen and stored at 80 C

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Chronic mTORC1 suppression generates a persistent phenotype

Other features include neurovascular structural damage caused by mechanical factors, secondary oedema formation, decreased cerebral perfusion levels, and increased glutamate levels (107110)