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Additionally, research has shown that 1 O 2 oxidizes mitochondrial ETC proteins, which results in defective mitochondrial respiration [49]

For fluorescence lifetime imaging of Flipper-TR (CY-SC020, Cytoskeleton) in neutrophils, cells were first stained with Hoechst 33342 (20 g ml 1 ), seeded in a four-chamber glass-bottom dish and treated with PMA (100 nM) at 37 C in a 5% CO 2 incubator for 2 h