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The pro-apoptotic members of this family, such as Bax and Bak, promote this permeabilization, releasing cytochrome c into the cytosol [221223]

The membranes were blocked with 5% nonfat milk in Tris-buffered saline-Tween 20 (TBST) for 1 h at room temperature before being incubated with primary antibodies against p16, p21 and GAPDH (all from Proteintech, China) overnight at 4 C

Dang L, Fan X, Chaudhry A, Wang M, Gaiano N, Eberhart CG

Another study showed transfection of cells with miR-133b, a microRNA targeting GSTP1, downregulated certain MMP protein expression and reduced cell migration in nonsmall cell lung cancer (Lin et al

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