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Mitochondrial glutathione, a key survival antioxidant Antioxid Redox Signal

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[CrossRef] [PubMed] [PubMed Central] [Google Scholar] Understanding Asthma phenotypes, endotypes, and mechanisms of disease

The media was changed every 48-72 hours by discarding the media, washing the cells either once (for J774A.1 cell lines) or three times (for Caco-2 cell lines) in pre-heated 1 x phosphate buffered saline (PBS), and subsequently adding new, pre-heated DMEM with appropriate supplements