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To test whether the protective effect of cysteine in ES2 and OVCAR3 cells is concentration-dependent, cells (5 10 5 cells/well) were seeded in 6-well plates and cultured in control condition and exposed to 0.1 mM, 0.2 mM, 0.4 mM, 0.8 mM and 1.0 mM L-cysteine and/or hypoxia induced with 0.1 mM cobalt chloride

All statistical analyses and graphics were made with GraphPad Prism 5.0 software (GraphPad Software Inc., San Diego, CA, USA)

doi: 10.3389/fonc.2020.625866 151 BatoolSSohailSUd DinFAlamriAHAlqahtaniASAlshahraniMAet al

Orotate (orotic acid): An essential and versatile molecule

Developing biomarkers reflecting mitochondrial status is another promising area for TBI research