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Detection of cell death For cell death assays, cells were washed twice in phosphate-buffered saline and then stained with Annexin V-FITC (Biovision, Mountainview, CA) and propidium iodide (PI, Sigma-Aldrich) according to the manufacturer's instructions

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This meticulous process significantly contributes to the inherent stability of our peptides, making your GHK-Cu storage efforts more effective from the outset