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Lane 1: Molecular weight marker Lane 2: Cell pellet after sonication Lane 3: Supernatant after sonication Lane 4: Flow through after binding Lane 5: Wash Lane 6: Elution 1 Lane 7: Elution 2 Lane 8: Elution 3 Lane 9: Elution 4 Lane 10: Elution 5 GST-E2-25K was purified with Glutathione Agarose Resin using a gravity column

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A coherent mechanistic rationale is not the same as demonstrated synergy, and animal-model effect sizes do not translate cleanly to human dosing

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However, Cagrilintide failed to induce significant cFos activation in RAMP1/3 KO mice, whereas sCT retained its ability to do so, likely via residual AMY2R signaling