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GHK reversed the gene expression pattern so it became consistent with the activation of the TGF-beta pathway

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However, some of the widely used affinity tags, such as the His-tag, exhibit several drawbacks and limitations, since they can increase the risk of distorting the natural conformation of the target protein or necessitate stringent elution and wash conditions affecting the yield of the target protein

Their exact causes remain elusive, as does a definitive cure

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