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To assess binding to a panel of RBD mutants, HIS1K biosensors (FortBio) were equilibrated in assay buffer (PBS) for 15 s before loading of His-tagged SARS-CoV-2 RBD, VoC RBDs, or SARS-CoV-1 RBD (30 g ml 1 diluted in PBS) for 100 s

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The decision framework considers multiple factors simultaneously - wrinkle severity (mild suggests peptides, severe suggests Botox), budget constraints (limited favors peptides, flexible allows Botox), lifestyle compatibility (busy schedules favor peptides, flexible timing allows Botox), aesthetic philosophy (natural appearance favors peptides, dramatic results favor Botox), age and prevention focus (younger favors peptides, established wrinkles favor Botox), and risk tolerance (conservative favors peptides, accepting side effects allows Botox) creating personalized recommendation rather than universal best choice

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