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Acta Orthop Scand 70:487490 Yeler H, Tahtabas F, Candan F (2005) Investigation of oxidative stress during fracture healing in the rats

Ketogenic diet aggravates colitis, impairs intestinal barrier and alters gut microbiota and metabolism in DSS-induced mice

Therefore, DHODH/CoQH 2 and mitochondrial GPX4 constitute two major ferroptosis defense systems to eliminate cell damage or death caused by the accumulation of mitochondrial lipid peroxides, and preclinical studies have shown that targeting DHODH can promote ferroptosis[36]

Following oral administration of 100 mg of flupirtine, clearance is 275 ml/min in healthy volunteers

Agents such as metformin, rapamycin, or trehalose can rejuvenate autophagy and mitophagy, thereby clearing damaged mitochondria and lipid droplets to reduce mitochondrial ROS and inflammatory priming

Briefly, protein extracts were prepared by homogenizing with a Polytron homogenizer (KINEMATICA AG, Switzerland) in lysis buffer containing: 20 mM HEPES (pH 7.9), 25% v/v glycerol, 0.42 M NaCl, 0.2 mM EDTA, 1.5 mM MgCl2, 0.5% v/v Nonidet P-40, 1 mM DTT, 1 mM Naf, 1 mM Na 3 VO 4 , and 1 complete protease inhibitor cocktail (Roche Diagnostics Ltd, Mannheim, Germany)