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K., Delaunay-Moisan, A., & Toledano, M

J Mol Biol 423: 475481

A comprehensive understanding of these processes within the Mediterranean basin is therefore essential for improving predictions of extreme weather events, including the recurrent severe floods and heatwaves affecting the region 5

The class I aaRSs feature a cytidylyltransferase-like Rossmann fold seen in proteins like glycerol-3-phosphate cytidylyltransferase, nicotinamide nucleotide adenylyltransferase and archaeal FAD synthase, whereas the class II aaRSs have a unique fold related to biotin and lipoate ligases

The samples were rotated at room temperature for 3 hours before washing with the 2 nd wash buffer (50 mM TEAB, 150 mM NaCl, 0.1% NP-40) 3 times, 3 times with PBS and 3 times with water

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