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Cells were seeded on collagen-coated plates (250 g/ml rat collagen, Roche) at a density of 150,000 cells/cm 2 and cultivated for 3 h in Williams E medium (PAN Biotech) containing penicillin (100 U/ml, Gibco), streptomycin (0.1 mg/ml, Gibco), gentamycin (10 M, PAN Biotech), dexamethasone (100 nM, Sigma), stable l-glutamine (2 mM, Sigma), insulin supplement (2 ng/ml, Sigma), and 10% Sera Plus (PAN Biotech)

(November 2019)

Gentric G, Kieffer Y, Mieulet V, Goundiam O, Bonneau C, Nemati F, et al

The impact of hyperfiltration on the diabetic kidney

The functions of the ascorbateglutathione cycle are powered by the pools of pyridine nucleotides, NAD(H) and NADP(H), as is ROS production by respiratory burst oxidase homologues (RBOHs) and other ROS-producing enzymes