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Oxygen regulates human pluripotent stem cell metabolic flux

Whereas autophagy can degrade large macromolecular complexes and even entire organelles, the UPS is characterized by exquisite substrate specificity, targeting only ubiquitinated proteins while preserving intact cellular structures

PMID 16189514

The resin was washed with buffer A supplemented with 20 mM imidazole, and the bound FSP1 proteins were eluted from the affinity resin with buffer A supplemented with 250 mM imidazole