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The novel advances in the biochemical diagnostics of primary aldosteronism, including omics techniques, presented in this review may help to address the most emerging problems, increase the number of diagnosed patients and facilitate the choice of an optimal treatment

doi: 10.1021/acs.cgd.7b00599 197 AliZSaleemMAttaBMKhanSSHammadG

XH: Conceptualization, Writing original draft

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The cDNA samples (20 ng) were used for RT-qPCR in a total volume of 10 L using SYBR Green Reagent (#04887352001, Roche Diagnostics, Meylan, France) and specific primers, on a LightCycler 96 Roche Instrument