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It chelated Cd in the cytosol and drove its secretion to extracellular spaces, promoting its loading into xylem vessels for root-to-shoot translocation and accumulation in leaves and straws, but not in rice grains ( NtCAL1 in tobacco functions as a positive factor in plant Cd accumulation and resistance ( NtCAL1 enhanced the content of ascorbic acid and the activities of antioxidant enzymes such as catalase (CAT) and ascorbate peroxidase (APX), helping to scavenge oxidative stress induced by Cd ( NtRAMP3, NtHMA, NtHMA ( AtPDF2.5 in Arabidopsis mediated Cd tolerance and accumulation (Luo et al., 2019), and its expression, which is induced by Cd, was primarily expressed in root xylem vascular bundles (Luo et al., 2020)

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The DNA-protein complexes were then electrophoretically transferred to Hybond-N membrane (Amersham)

2018;7:e35136

Aging Cell 16 , 761772 (2017)

Orange (Citrus Sinensis ) essential oil Orange essential oil has strong antioxidant and anti-aflatoxin B activity against fungal toxins and antimicrobial activity