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When the balance between these reactive species and the body's antioxidant defenses shifts, oxidative stress occurs

Acknowledgements The authors thank the research resource division (RRD) (RIKEN Center for Brain Science) and RIKEN CBS-EVIDENT Open Collaboration Center (RIKEN Center for Brain Science) for technical assistance, Yukiko Nagai (RIKEN Center for Brain Science) for secretarial work, Kenichi Nagata (Nagoya University) and members of the Laboratory for Proteolytic Neuroscience (RIKEN Center for Brain Science) for beneficial suggestions

Science 293 , 18321836 (2001)

It is accepted that an excessive burden of ROS may lead to abnormal cell growth, corresponding changes in intracellular homeostasis and damage to important components of in the cell when the antioxidant defense and/or DNA repair mechanisms cannot balance the excess oxidants [29]

Cysteine levels in the supernatant were measured by a Cysteine Assay Kit (#MAK255, Sigma) and the cysteine standards

10.3389/fpls.2020.560768 Front