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PMID 36242844

It was observed that nFhGST significantly suppressed the LPS-induced TNF and IL1 in vitro production by macrophages and the pro-inflammatory cytokine/chemokine storm within C57BL/6 mice elicited by lethal doses of LPS (84)

At the end of the intervention period, animals underwent 12-hour fasting

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However, no study has delineated the proteinprotein interaction (PPI) and metabolic-protein interactions (MPIs) networks of CFAS-D and the biological processes, molecular functions and complexes, cellular components, pathways, transcriptional regulatory relationships, protein domains, and human disease annotations, which are associated with the PPI and MPI networks of CFAS-D

Notably, histones and other NET components function as DAMPs, further amplifying NLRP3 inflammasome activation and pyroptosis in macrophages, thereby perpetuating a vicious inflammatory cycle ( Critically, direct interplay exists between pyroptosis and NETosis: membrane pores formed by GSDMD activate neutrophil elastase (NE), which in turn cleaves GSDMD to promote NETosis ( Apoptosis In contrast to the pro-inflammatory properties of pyroptosis and NETosis, PCD in the form of apoptosis acts in an immunologically silent manner and plays a critical regulatory role during the resolution phase of GA inflammation