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Therapeutic strategies targeting the gut microbiota include conventional probiotics, prebiotics, and fecal microbiota transplantation, alongside emerging synthetic biology approaches employing genetically engineered probiotic strains to deliver bioactive molecules, including GLP-1, directly in the gut microenvironment

The slides were then stained with a standard tertiary method: incubated with indicated primary antibodies (TOM20 (Abcam, ab186734

Recent reviews and empirical studies have demonstrated that CRISPR/Cas, recombineering, and heterologous-expression toolkits enable the precise engineering of lactic acid bacteria and other probiotic chassis (Mugwanda et al., 2023