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Ordinal scales, such as ALSFRS-R, where total sum scores are compared within participants, sometimes under- or over-value changes (i.e., the difference in scores in patients who go from walking up stairs to using a handrail is considered equivalent to the difference between feeding oneself and relying on others for all feeds)

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Primers for SOD1, CAT, GPx, and GAPDH were constructed as previously described 23