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Total RNA was then used to synthesize complementary DNA, followed by the performance of real-time PCR using an SYBR Green I technology and MxPro-Mx3000P QPCR machine

Adverse events were mild to moderate in severity, most commonly gastrointestinal disturbance (207 adverse events, 37%), with similar rates across treatment groups

For researchers dealing with autoimmune conditions or chronic inflammatory states, this modulation rather than suppression mechanism is particularly relevant