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This effect was supported by a colony formation assay, where treatment with CBD, 19 , and 21 at concentrations near their IC 50 (i.e., 22, 7, and 5 M

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When a frameshift mutation replaces the IDR acidic tail of HMGB1 with an arginine-rich basic tail, HMGB1 with a mutant tail undergoes LLPS to form droplets at a lower concentration, attenuates the dynamic and liquid fluidity of HMGB1 droplets, and facilitates HMGB1 partitioning into nuclear condensates, culminating in mispartitioning into the nucleolus

10.1093/cvr/cvu050 325 ZhangX.ZhuJ.-X.MaZ.-G.WuH.-M.XuS.-C.SongP.et al (2019)