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Before incubation with the primary antibody, lipid droplets in bovine oocytes were cleared with 4,000 U/ml lipase from Candida rugose (Thermo Fisher Scientific) in 400 mM NaCl, 50 mM Tris (pH 7.2), 5 mM CaCl 2 , and 0.2% sodium taurocholate supplemented with ethylenediaminetetraacetic acid-free Protease Inhibitor Cocktail (11,836,170,001

The color of the EV-QuerCar formulation remained almost unchanged

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Furthermore, consumption of GT significantly decreased serum PSA ( P = 0.04)

Classes were then subjected to non-uniform (NU) refinement (initial lowpass resolution = 15 ), followed by local refinement masking out the density corresponding to the micelle