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Int J Oncol (2000) 16:12159

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Polysaccharide from Angelica sinensis protects H9c2 cells against oxidative injury and endoplasmic reticulum stress by activating the ATF6 pathway

Because high ionic strength can affect the stability of some proteins [40], weak exchangers might be more suitable for proteins that do not require an extreme pH for binding

doi: 10.3389/fchem.2020.583272 Received 14 July 2020 Accepted 13 October 2020 Published 12 November 2020 Volume 8 - 2020 Edited by Chun Xu, The University of Queensland, Australia Reviewed by Junfeng Zhao, Jiangxi Normal University, China

The membranes were then rinsed with PBS three times (5 min each), followed by an incubation in blocking solution (30% Roti Block (Carl Roth, Karlsruhe, Germany)) in Tris-Buffered Saline with 0.1% Tween 20 (TBST