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Immunomodulation by vitamin B12: augmentation of CD8 + T lymphocytes and natural killer (NK) cell activity in vitamin B12-deficient patients by methylB12 treatment

Liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based quantitative methods are one of the most commonly used techniques, with the advantage of being able to efficiently separate and detect a variety of carnitine and its derivatives, including free carnitine, acylcarnitine, and their isomers and odd forms

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After blocked with 3% BSA in Tris-HCl (50 mmol/L Tris, pH 7.4, 200 mmol/L NaCl) buffer solution containing 0.1% Tween-20 for 4 h at room temperature, the membranes were incubated overnight at 4 C with specific antibodies against protein kinase B (AKT

Co-chairs were J.K.B