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Discard if color shifts from pale blue to brown or green, which indicates copper speciation change

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If you think you will be purifying the same exact protein again in the future, then you should rinse your elution buffer out with a few column volumes of deionized water (dIH 2 O), and then store the beads in 20% ethanol until youre ready to use them again

Synthesis and biological evaluation of EC20: a new folate-derived, (99m)Tc-based radiopharmaceutical

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However, as with any potent wellness tool, questions about its safetyparticularly concerning heart healthhave surfaced in recent years