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Previous studies have shown that a reduced nucleotide pool and nucleotide imbalances favor DNA damage [44]

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21,32 In addition, iron is important for normal mitochondrial function, ER stress, DNA impairment and repair, and other cell survival-related enzymatic reactions

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ICIE offers a robust, synergistic platform that combines cryosurgery, controlled drug and gene delivery, and immune regulation to address the shortcomings of traditional cancer treatments, presenting a viable avenue for the treatment of aggressive and metastatic malignancies (Fig