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Oxidative stress and altered antioxidant defenses in children with acute exacerbation of atopic dermatitis

doi:10.1515/pjvs-2016-0074 Woolf EK, Terwoord JD, Litwin NS, et al

Step 2: Start with a Small Dose

Lai, S.-W

Commercially available RNase A, purified by HPLC was unfolded in a 6 M guanidinium hydrochloride (GuHCl) denaturating buffer using tris(2-carboxyethyl)phosphine (TCEP) as reducing agent