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CD38 is an ectoenzyme (NADase) whose expression increases with age and chronic inflammation

We used human adipocytes as the model system and show that vitamin B 12 restriction induced cholesterol biosynthesis in adipocytes by limiting cellular AdoMet levels and modulating the methylation of genes which regulate cholesterol biosynthesis

(Takahashi et al., 2022) observed an elevation in renal NNMT expression and a reduction in NAD + levels

This higher dose can be administered as a single 100mg injection in the morning or split into two 50mg doses taken 12 hours apart (morning and afternoon)