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Cells were seeded at a density of 2.7 10 4 cells/cm 2 in culture flasks and were cultured in Williams Medium E (GIBCO, Darmstadt, Germany) containing 10% (v/v) fetal calf serum (FCS) (Capricorn Scientific, Ebsdorfergrund, Germany), 2 mM L-glutamine (Thermo Fisher Scientific, Darmstadt, Germany), 5 g/mL insulin (Sigma-Aldrich, Steinheim, Germany), 50 M hydrocortisone-hemisuccinate (Sigma-Aldrich) and 1X antibiotic antimycotic solution (AAS) (Sigma-Aldrich)