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Cell Probes 30 , 386396 (2016)

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Using strain HA03, we further examined hyaluronic acid production following the overexpression of the genes that supply the precursors, N -acetyl glucosamine ( glmS, glmM , and glmU ) and UDP-glucuronic acid ( galU and ugd )

Microsomes were prepared by sonication of 10 8 cells, removal of debris by centrifugation at 10,000 g for 15 min, and subsequent centrifugation of the resulting supernatant at 100,000 g for 60 min

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