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After another centrifugation step for 15 min at 20,000 g and 4 C, 5x Laemmli buffer containing 10% -mercaptoethanol (-Me) was added to the samples

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Several authors have shown that high doses of vitamin E can also interfere with drug metabolism ( In the HOPE study, supplementation of 400 IU (=268 mg) of vitamin E/day in mild to moderate CKD patients has no effect on cardiovascular outcomes and overall mortality and therefore supplementation of vitamin E is not recommended (HOPE Study Investigators, 2000)

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