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Lactobacillus plantarum L15 alleviates colitis by inhibiting LPS-mediated NF-B activation and ameliorates DSS-induced gut microbiota dysbiosis

Sample processing and mass spectrometry analysis: Captured protein samples undergo strict enzymatic digestion and purification before high-resolution LC-MS/MS analysis to identify proteins and provide comprehensive qualitative and quantitative data

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All these alterations contribute not only to the weakening of the response to conventional therapy in this population, but also to chronotherapy, which presupposes a physiological rhythmicity that is not observed in these patients, particularly in the presence of comorbidities, polypharmacy, or sleep disorders (Lvi et al., 2010