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Ergothioneine Stimulates Ca2+-Mediated Brain-Derived Neurotrophic Factor Expression in NE-4 C Nerve Cells

HPLC Verification of All Four Components Because KLOW contains four peptides in a single vial, verification requires confirming the presence and quantity of each component independently rather than simply measuring total peptide content

After centrifugation at 20,000 g for 1 h, supernatants were incubated with Ni 2+ Sepharose and applied to two successive washes with buffer A (50 mM Tris pH 7.5, 1.2 M KCl, 20 mM imidazole) and buffer B (50 mM Tris pH 7.5, 150 mM NaCl)

Draw the desired amount of sterile water into a syringe (often 2-5mL depending on desired level)

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Vortexing creates shear and foam that denature peptides and dissociate the GHK-Cu chelate