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Thanks to the scientific research service platform of Scientific Research Center of China-Japan Union Hospital of Jilin University for supporting this study

Biochem J (2001) 360:55762

Following Hg exposure, the red blood cell (RBC) to plasma Hg concentration ratio typically ranges between 1:1 and 2:1 (WHO, 1991)

For oral sprays , dosing tends to run slightly higher: 500-1000mcg daily, again often split between morning and evening

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The day before the assay, Seahorse probe plates were prepared by adding 200 l distilled water, and calibration buffer was added to a 50 ml Falcon tube