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Increased production and accumulation of A in lysosomes has been observed in autophagy-deficient cells, suggesting that the turnover portion of A is regulated by autophagy

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Proteins were resolved through SDS-PAGE, transferred to PVDF membranes and probed with the specific antibodies indicated in the Western blotting section

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From this solution AC (17.6 mm), another solution prepared with using of sterile PBS.2 mL of solution AC plus 2 mL PBS used for preparation of solution BC (8.8 mM) and then 2 mL of solution BC plus 2 mL PBS used for making of solution CC (4.4 mM) and finally 2 mL of solution CC plus 2 mL PBS mixed for preparation of solution DC (2.2 mM)