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Inhibitory effect of gallic acid on advanced glycation end products induced up-regulation of inflammatory cytokines and matrix proteins in H9C2 (2-1) cells

[DOI] [PMC free article] [PubMed] [Google Scholar] 40.Fang R.H., Hu C.-M.J., Luk B.T., Gao W., Copp J.A., Tai Y., OConnor D.E., Zhang L

Abstract Longevity medicine is an emerging and iterative healthcare discipline focusing on early detection, preventive measures, and personalized approaches that aim to extend healthy lifespan and promote healthy aging

Mitochondrial membrane potential (m) assay The m was measured as previously described [39]

For activity screening, we adapted a GSTO1 substrate assay that relies on the quantification of the reduction of S -(4-nitrophenacyl)glutathione (4-NPG), a GSTO1-specific substrate 17 , using recombinant human GSTO1 in a high-throughput screening format