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Untargeted metabolomics analysis Fecal specimens (50 mg) were homogenized with a methanol-acetonitrile-water (2:2:1, v/v/v) extraction solvent using an ultrasonic-assisted extraction method, followed by protein precipitation and centrifugal separation

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Biochemistry 56:32993306 Ciosk R, Shirayama M, Shevchenko A, Tanaka T, Toth A, Shevchenko A, Nasmyth K (2000) Cohesins binding to chromosomes depends on a separate complex consisting of Scc2 and Scc4 proteins

When symptoms are attributed to high B12 intake, they typically include: Skin reactions : Acne-like eruptions or rosacea-type rashes, particularly with hydroxocobalamin injections (listed as rare adverse effects in the product information) Gastrointestinal disturbances : Mild nausea, diarrhoea, or abdominal discomfort Headaches : Occasional reports of headache following B12 injections Anxiety or restlessness : Anecdotal reports, though causation remains unestablished It is crucial to distinguish between symptoms caused by elevated B12 levels and those arising from the underlying conditions that elevate B12

Tukeys HSD and Dunnett-Hsu post-hoc test were used to compare performance in the T-maze task