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Tris(hydroxymethyl)aminomethane (Tris) buffer

Murphy MP, Bayir H, Belousov V, Chang CJ, Davies KJA, Davies MJ et al (2022) Guidelines for measuring reactive oxygen species and oxidative damage in cells and in vivo

Those findings imply that while the reduced GSH levels in AS NPCs contribute to the elevated mROS, the aberrant m levels may derive from other AS mitochondria-related factors, such as structural aberrations and malfunction of the inner mitochondrial membrane or its respiratory complexes

In one assay, inhibition of ChaC1 prevents growth

Interestingly, the incubation with the antioxidant N-acetylcysteine (NAC) upon treatment with NP4 resulted in reduced levels of apoptosis, suggestive that the cytotoxicity of NP4 is related to intracellular redox homeostasis (Supplementary Fig

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