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RNA samples were further processed for mRNA-seq library preparation according to the manufacturers instructions (TruSeq RNA Sample preparation v2, Illumina, San Diego, CA, USA)

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Unlike free lipid peroxidation products such as MDA or 4-HNE, ox-LDL circulates systemically and engages in pathogenic signaling by activating Kupffer cells, promoting NLRP3 inflammasome activation, and inducing proinflammatory cytokines like TNF- and IL-1 [151, 152]

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