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Annals of the NY Academy of Sciences , 1194, 179189

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The further steps included incubation of specimens in 2% osmium tetroxide (Sigma-Aldrich) for 2 h at room temperature and dehydration in a series of ethanol solutions (Linegal Chemicals, Poland), starting from 10 min twice in 50 and 70%, followed by 5 min twice in 95% and 1 min twice in 100% ethanol solution